Heat stress transcription factor DcHsfA1d isolatedfrom Dianthus caryophyllus enhances thermotoleranceand salt tolerance of transgenic Arabidopsis

Heat shock transcription factors (Hsfs) participate in a variety of plant physiological processes including the regulation associated with thermotolerance. Here, Hsf gene DcHsfA1d was identified from carnation (Dianthus caryophyllus L.). The open reading frame (ORF) 1 368 bp and encoded protein 455 amino acids molecular mass 51.039 kDa an isoelectric point 4.94. Sequence domain prediction revealed that exhibited five typical functional features motifs. significantly up-regulated under heat stress or ABA treatment. Yeast two-hybrid experiment indicated DcHsp70 physically interact each other. Overexpression Arabidopsis ecotype Columbia enhanced seedling thermotolerance by increasing activities catalase, peroxidase, superoxide dismutase while reducing relative electrolyte leakage, malondialdehyde content, accumulation O2- H2O2 initiating transcriptional thermal protective expression stress. Furthermore, salt stress, root length fresh ectopically expressing were higher than those wild type, which tolerance transgenic improved to certain extent. In summary, demonstrated play positive regulatory role response it might be candidate for using genetic modification.